School of Medicine

Wayne State University School of Medicine

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Abdul B. Abou-Samra, M.D., Ph.D.
Professor of Medicine and Chief, Division of Endocrinology
University Health Center - 4H
4201 St. Antoine
Detroit, MI 48201
313-745-4008
asamra@med.wayne.edu
Biography

Education:

  • M.D., Aleppo's University 1979
  • Ph.D., Biochemistry, Lyon 1 University 1983

Positions and Employment:

  • 1979-1984 - Resident and Fellow, Internal Medicine and Endocrinology, Hôpital de l’ Antiquaille, Lyon, France /  Université Claude Bernard (Lyon I) Medical School, Lyon France.
  • 1984-1986 - Visiting Fellow, Endocrinology and Reproductive Research Branch, NICHD, NIH, Bethesda, Maryland.
  • 1986-1988 - Fellow in Endocrinology, Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston MA
  • 1988-2007 - Staff Physician, Massachusetts General Hospital, Boston, MA
  • 1986-1988 - Instructor in Medicine, Harvard Medical School, Boston, MA
  • 1988-1993 - Assistant Professor of Medicine, Harvard Medical School, Boston, MA
  • 1993-2007 - Associate Professor of Medicine, Harvard Medical School, Boston, MA
  • 2007-Present - Professor of Medicine, Wayne State University, Detroit, MI
  • 2007-Present - Chief, Division of Endocrinology and Metabolic Diseases, Wayne State University School of Medicine, Detroit, MI

Honors:

  • 1983 - Research Award, “Foundaton la Recherch Medicale”, Paris, France, 1984
  • 1984 - Maitre-Es-Science Medical, Lyon I University, Lyon France
  • 1994-2002 - Subcommittee of Study of Research Proposals, Mass General Hospital, Boston, MA
  • 2001 - Partners of Excellence Award
  • 2001-2003 - Endocrinology Study Section Center of Scientific Review, NIH
  • 2001-2004 - Editorial Board, Endocrinology
Joint Appointments
Center of Molecular Medicine and Genetics, Karmanos Cancer Institute
Research

Ongoing Research Support:

  • Principal Investigator,  R01 DK063211  (NIH/NIDDK) 1/15/03 - 12/31/09
    Role of PTH1R in bone biology using a phosphorylation-deficient PTH1R knock-in mouse model The goal of this project is to study the role of the phosphorylation of the PTH/PTHrP receptor in the physiological actions of PTH in vivo using a knock-in mouse model expressing a PTH1R in which the phosphorylation sites were mutated.

Previous Research Support (completed projects):

  • Principal Investigator,  R01 DK063211-01A1 (NIH/NIDDK)  7/1/2003 - 10/31/2008
    Bi-molecular interactions of the CRF ligands and receptors
    The goal of this project is to study the interactions between corticotropin-releasing factor (CRF), urocortin-1 and sauvagine with the CRF receptors.
  • Principal Investigator, SubProject II of P01 DK11794 (Potts, Program Director) 12/1/1993-11/30/2008
    Subproject II: "Regulation of the PTH/PTHrP receptor" of Program Project "Hormonal Control of Calcium Metabolism"
    The goals of this project are to characterize the post-translational mechanisms of desensitization and internalization of the PTH/PTHrP receptor.
  • Principal Investigator, R01DK045485 (NIH/NIDDK)   3/1/1995 - 2/29/2000
    Structure and Regulation of the PTH/PTHRP Receptor Gene
    The goals of this project are to clone and characterize the transcriptional regulation of the PTH/PTHrP receptor gene.
  • Principal Investigator, 5R01DK011794 (NIH/NIDDK) 1/1/1990 - 12/31/1995
    CRF Receptors- Structure, Function Expression
    The goals of this project are to clone the receptors for corticotropin releasing factor (CRF) and characterize its binding and signaling properties.
Peer-Reviewed Publications
  1. Abou-Samra AB, Estour B, Bajard L, Tourniare J. Insulin a la pompe: Interet dans l'adaptation du traitement des diabetes difficiles a controler. Lyon Med. 1984; 242:393-398.
  2. Abou-Samra AB, Fevre-Montange M, Pugeat M, Dechaud H, Chalendar D, Estour B, Tourniaire J. The value of beta-lipotrophin measurement during the short metyrapone test in patients with pituitary diseases and in Cushing's syndrome. Acta Endocrinol. 1984; 105:441-448.
  3. Abou-Samra AB, Loras B, Pugeat M, Tourniaire J, Bertrand J. Demonstration of an antiglucocorticoid action of progesterone on the corticosterone inhibition of beta-endorphin release by rat anterior pituitary in primary culture. Endocrinology. 1984; 115: 1471-1475.
  4. Abou-Samra AB, Pugeat M, Dechaud H, Nachury L, Bouchareb B, Fevre-Montange M, Tourniaire J. Increased plasma concentrations of N-terminal beta-lipotropin and unbound cortisol in pregnancy. Clin Endocrinol. 1984; 20:221-228.
  5. Abou-Samra AB, Pugeat M, Dechaud H, Nachury L, Tourniaire J. Acute dopaminergic blockade by sulpiride stimulates beta-endorphin secretion in pregnant women. Clin Endocrinol. 1984; 21:583-588.
  6. Fevre-Montage M, Estour B, Abou-Samra AB, Bajard L, Tourniaire J. 24 hour melatonin secretory pattern in idiopathic hemochromatosis. Psycho-Neuroendocrinol. 1983; 8:321-326.
  7. Abou-Samra AB, Dechaud H, Estour B, Chalendar D, Fevre-Montange M, Pugeat M, Tourniaire J. Beta-lipotropin and cortisol responses to an intravenous infusion dexamethasone suppression test in Cushing's syndrome and obesity. J Clin Endocrinol Metab. 1985; 61:116-119.
  8. Abou-Samra AB, Fevre-Montange M, Loras B, Durand A, Tourniaire J, Bertrand J. Effect of indolamines on beta-endorphin release by rat anterior pituitary cells. Neuroendocrinology. 1985; 41:490-493.
  9. Tourniaire J, Fevre-Montange M, Abou-Samra AB, Dechaud H. Melatonine et fonction corticotrope. Bull Acad Natl Med. (Paris). 1986; 170: 803-809.
  10. Abou-Samra AB, Catt KJ, Aguilera G. Biphasic inhibitory effect of corticosterone on stimulated ACTH release in rat anterior pituitary cell cultures. Endocrinology. 1986; 119:972-977.
  11. Abou-Samra AB, Catt KJ, Aguilera G. Involvement of protein kinase C in the control of ACTH secretion in rat anterior pituitary cell cultures. Endocrinology. 1986; 118:212-217.
  12. Abou-Samra AB, Catt KJ, Aguilera G. Role of arachidonic acid in the regulation of ACTH release from rat anterior pituitary cell cultures. Endocrinology. 1986; 119:1427-1431.
  13. Cohen H, Sabbagh I, Abou-Samra AB, Bertrand J. Beta-endorphin in genetically hypoprolactinemic rat: IPL nude rat. Life Sci. 1986; 38:217-224.
  14. Tourniaire J, Chalendar D, Rebatu B, Fevre-Montange M, Bajard L, Mazenod B, Dechaud H, Abou-Samra AB, Cauter EV. The 24 hour cortisol secretory pattern in Cushing's syndrome. A quantitative analysis. Acta Endocrinol. 1986; 112:230-237.
  15. Tourniaire J, Fevre-Montange M, Abou-Samra AB, Dechaud H. Melatonin and the corticotropic function. Bull Acad Natl Med 1986; 170:803-809.
  16. Abou-Samra AB, Catt KJ, Aguilera G. Atrial natriuretic factor stimulates cyclic GMP production and LH release in rat anterior pituitary cells: contamination with GnRH explains its LH releasing activity. Endocrinology. 1987; 120:18-24.
  17. Abou-Samra AB, Catt KJ, Aguilera G. Calcium dependent control of ACTH secretion in rat anterior pituitary cells. Endocrinology. 1987; 121:965-971.
  18. Abou-Samra AB, Harwood J, Manganellio VC, Catt KJ, Aguilera G. Phorbol 12-myristate 13-acetate and vasopressin potentiate the effect of CRF on cyclic AMP production in rat anterior pituitary cells: mechanisms of action. J Biol Chem. 1987; 262:1129-1136.
  19. Milan M, Abou-Samra AB, Catt KJ, Aguilera G. Receptors and actions of CRF receptors in the primate pituitary gland. J Clin Endocrinol Metab. 1987; 64:1036-1041.
  20. Aguilera G, Abou-Samra AB, Harwood JP, Catt KJ. Corticotropin-releasing factor receptors: characterization and action in the anterior pituitary gland Adv Exp Med Biol. 1988; 245:83-95.
  21. Jüppner H, Abou-Samra AB, Uneno S, Gu WX, Potts JT Jr, Segre G. The parathyroid hormone-like peptide associated with humoral hypercalcemia of malignancy and parathyroid hormone bind to the same receptor on the plasma membrane of ROS 17/2.8 cells. J Biol Chem. 1988; 263:8557-8560.
  22. Abou-Samra AB, Jüppner H, Potts JT Jr, Segre GV. Inactivation of pertussis toxin-sensitive guanyl nucleotide-binding proteins increase parathyroid hormone receptors and reverse agonist-induced receptor down-regulation in ROS 17/2.8 cells. Endocrinology. 1989; 125:2594-2599.
  23. Abou-Samra AB, Jüppner H, Westerberg DP, Potts JT Jr, Segre GV. Parathyroid hormone causes translocation of protein kinase-C from cytosol to membranes in rat osteosarcoma cells. Endocrinology 1989; 124:1107-1113.
  24. Abou-Samra AB, Uneno S, Jüppner H, Keutmann H, Potts JT Jr, Segre GV, Nussbaum SR. Non-homologous sequences of parathyroid hormone and the parathyroid hormone related peptide bind to a common receptor on ROS 17/2.8 cells. Endocrinology. 1989; 125:2215-2217.
  25. Abou-Samra AB, Freeman M, Jüppner H, Uneno S, Segre GV. Characterization of fully active biotinylated parathyroid hormone analogs: Application to fluorescence-activated cell sorting of parathyroid hormone receptor bearing cells. J Biol Chem. 1990; 265:58-62.
  26. Gardella TJ, Rubin D, Abou-Samra AB, Keutmann HT, Potts JT, Kronenberg HM, Nussbaum SR. Expression of human parathyroid hormone (1-84) in Escherichia coli as a factor x-cleavable fusion protein. J Biol Chem. 1990; 265:15854-15859.
  27. Jüppner H, Abou-Samra AB, Uneno S, Keutmann HT, Potts JT Jr, Segre GV. Preparation and characterization of [N(4-Azido-2-nitrophenyl)Ala1,Tyr36] parathyroid hormone related peptide (1-36) amide: A high-affinity, partial agonist having high cross-linking efficiency with its receptor on ROS 17/2.8 cells. Biochemistry. 1990; 29:6941-6946.
  28. Jüppner H, Abou-Samra AB, Uneno S, Schipani E, Keutmann HT, Potts JT Jr., Segre GV. Properties of amino terminal parathyroid hormone-related peptides modified at positions 11-13. Peptide. 1990; 11:1139-1142.
  29. Abou-Samra AB, Zajac J, Schiffer-Alberts D, Skurat R, Kearns A, Segre GV, Bringhurst FR. Cyclic adenosine 3',5'-monophosphate (cAMP)-dependent and cAMP-independent regulation of parathyroid hormone receptors on UMR 106-01 osteoblastic osteosarcoma cells. Endocrinology 1991; 129: 2547-2554.
  30. Bergwitz C, Madoff S, Abou-Samra AB, Jüppner H. Specific, high-affinity binding sites for angiotensin II on mycoplasma hyorhinis. Biochem Biophys Res Comm. 1991; 179: 1391-1399.
  31. Jüppner H, Abou-Samra AB, Freeman M, Kong XF, Schipani E, Richards J, Kolakowski LF Jr, Hock J, Kronenberg HM, Segre GV. A G protein-linked receptor for parathyroid hormone and parathyroid hormone-related peptide. Science 1991; 245:1024-1026.
  32. Abou-Samra AB, Jüppner H, Force T, Freeman MW, Kong XF, Schipani E, Urena P, Richards J, Bonventre JV, Potts JT Jr, Kronenberg HM, Segre GV. Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: A single receptor stimulates intracellular accumulation of both cAMP and inositol trisphosphates and increases intracellular free calcium. Proc Natl Acad Sci USA. 1992; 89:2732-2736.
  33. Uneno S, Yamamuro T, Jüppner H, Abou-Samra AB, Keutmann HT, Potts JT Jr, Segre, GV. Solubilization of functional receptors for parathyroid hormone and parathyroid hormone-related peptide from clonal rat osteosarcoma cells ROS 17/2.8. Calcif Tiss Int. 1992; 51:382-386.
  34. Tojo K, Abou-Samra AB. Corticotropin-releasing factor (CRF) stimulates 45Ca2+ uptake in the mouse corticotroph cell line AtT-20. Life Science 1992, 52:621-630.
  35. Abou-Samra AB, Jüppner H, Khalifa A, Karga H, Kong XF, Schiffer-Alberts D, Xie LY, Segre GV. Parathyroid hormone (PTH) stimulates adrenocorticotropin release in AtT-20 cells stably expressing a common receptor for PTH and PTH-related peptide. Endocrinology 1993; 132:801-805.
  36. deStolpe AV, Karperien M, Löwik CWGH, Jüppner H, Segre GV, Abou-Samra AB, deLaat SW, Defize LHK. Parathyroid hormone-related peptide as an endogenous inducer of parietal endoderm differentiation. J Cell Biol 1993; 120:235-243.
  37. Schipani E, Karga H, Karaplis AC, Potts JT Jr, Kronenberg HM,Segre GV, Abou-Samra AB, Jüppner H. Identical cDNAs encode a human renal and bone PTH/PTHrP receptor. Endocrinology 1993; 132:2157-2165.
  38. Bringhurst FB, Jüppner H, Kronenberg HMK, Abou-Samra AB, Segre GV. Cloned, stably expressed PTH/PTHrP receptors activate multiple messenger signals and biological responses in LLCPK-1 kidney cells. Endocrinology 1993; 132:2090-2098.
  39. Lee K, Bond AT, Jüppner H, Abou-Samra AB, Segre GV. In situ hybridization of PTH/PTHrP receptor mRNA in the bone of fetal and young rats. Bone 1993; 14:341-345.
  40. Urena P, Kong XF, Abou-Samra AB, Jüppner H, Kronenberg HK, Potts Jr JT, Segre GV. Parathyroid hormone (PTH)/PTH-related peptde receptor mRNA is widely distributed in rat tissues. Endocrinology 1993; 133:617-623.
  41. Urena P, Iida-Klein A, Kong XF, Jüppner H, Kronenberg HM, Abou-Samra AB, Segre GV. Regulation of the parathyroid homrone (PTH)/PTH-related peptide receptor mRNA by glucocorticoids and PTH in ROS 17/2.8 and OK cells. Endocrinology 1994; 134:451-456.
  42. Gwosdow AR, O'Connell NA, Spencer JA, Kumar MSA, Agarwal RK, Bode HH, Abou-Samra AB. Interleukin-1 induced corticosterone release occurs by an adrenergic mechanism from the rat adrenal gland. Am J Physiol.1992; 263:E461-E466.
  43. Gwosdow AR, Spencer JA, O'Connell NA, Kraytsberg G, Abou-Samra AB. Interleukin-1 activates protein kinase A and stimulates adrenocorticotropic hormone release from AtT-20 cells. Endocrinology 1993; 132:710-714.
  44. Gwosdow AR, O’Connell NA, Abou-Samra AB. Interleukin-1 increases protein kinase A activity by a cAMP-independent mechanism in AtT-20 cells. Am J Physiol 1994; 266: E79-E84.
  45. Jüppner H, Schippani E, Bringhurst RF, McClure I, Keutmann HT, Potts Jr JT, Kronenberg HM, Abou-Samra AB, Segre GV, Gardella TJ. The extracellular amino-terminal region of the PTH/PTHrP receptor determines the binding specificity for the carboxy-terminal fragments of PTH(1-34). Endocrinology, 1994, 134:879-884.
  46. Okano K, Wu S, Huang X, Pirola CJ, Jüppner H, Abou-Samra AB, Segre GV, Iwasaki K, Fagin JA, Clemens TL. Parathyroid hormone (PTH)/PTH-related protein (PTHrP) receptor and its messenger RNA in rat aortic smooth muscle cells and UMR osteoblast-like cells: cell-specific regulation by angiotensin-II and PTHrP Endocrinology 1994, 135:1093-1099.
  47. Fukayama S, Schipani E, Jüppner H, Lanske B, Kronenberg HM, Abou-Samra AB, Bringhaurst FR. Role of protein kinase-A (PKA) in homologous down-regulation of PTH/PTHrP receptor mRNA in human osteoblast-like cells. Endocrinology 1994, 134:1851-1858.
  48. Urena P, Kubrusly M, Mannstadt M, Hruby M, Tan MMTT, Silve C, Lacour B, Abou-Samra AB, Segre GV, Drueke T. The renal PTH/PTHrP receptor is down-regulated in rats with chronic renal failure. Kidney International 1994, 45:605-611.
  49. O’Connell NA, Kumar A, Chatzipanteli K, Mohan A, Agarwal RK, Head C, Bornstein S, Abou-Samra AB, Gwosdow AR. Interleukin-1 regulates corticosterone secretion from the rat adrenal gland through a catecholamine-dependent and prostaglandin E2-independent mechanism. Endocrinology, 1994; 135:460-467.
  50. Lee CW, Gardella TJ, Abou-Samra AB, Nussbaum SR, Segre GV, Potts JTP Jr., Kronenberg HM, Juppner H. Role of the extracellular regions of the PTH/PTHrP receptor in hormone binding. Endocrinology 1994, 135:1488-1495.
  51. Gardella TJ, Jüppner H, Wilson AK, Abou-Samra AB, Segre GV, Bringhaurst FR, Potts Jr. JTP, Nussbaum SR, Kronenberg HM. Determinants of [Arg2]-PTH binding and signaling in the transmembrane region of the parathyroid hormone recetpor. Endocrinology 1994, 135:1186-1194.
  52. Kong X-F., Schipani E, Lanske B,Joun H, Karperien M, Defize LHK, Jüppner H, Potts JTJr., Segre GV, Kronenberg HM and Abou-Samra AB. The rat, mouse and human genes encoding the receptor for parathyroid hormone and parathyroid hormone-related peptide are highly homologous. Biochem Biophys Res Comm 1994, 200:1290-1299.
  53. Abou-Samra AB, Goldsmith P, Xie LY, Jüppner H, Spiegel A, Segre GV Regulation of the PTH/PTHrP receptor immunoreactivity and PTH binding in opossum kidney cells by PTH and dexamethasone. Endocrinology 1994, 135:2588-2594.
  54. Urena P, Mannstadt M, Hruby M, Ferreira A, Schmitt F, Silve C, Ardaillou R, Lacour B, Abou-Samra AB, Segre GV, Drueke T. Parathyroidectomy does not prevent the renal PTH/PTHrP receptor down-regulation in uremic rats. Kidney International 1995; 47:1797-1805.
  55. McCauley LK, Beecher CA, Melton ME, Werkmeister JR, Jüppner H, Abou-Samra AB, Segre GV,Rosol TJ. Transforming grouth factor-b1 regulates steady-state PTH/PTHrP receptor mRNA levels and PTHrP binding in ROS 17/2.8 osteosarcoma cells. Mol Cell Endocrinol 1994, 101:331-336.
  56. Karperien M, Van Dijk TB, Hoeijmakers T, Cremers F, Abou-Samra AB, Boonstra J, deLaat SW, Defize LHK. Expression pattern of parathyroid hormone/parathyroid hormone related peptide receptor mRNA in mouse postimplantation embryos indicates involvement in multiple developmental processes. Mechanism of Development 1994, 47:29-42.
  57. Bergwitz C, Abou-Samra AB, Hesch RD, Jüppner H. Rapid desensitization of parathyroid hormone-dependent adenylate cyclase in perifused human osteosarcoma cells (SaOS-2). Biochem Biophys Acta. 1994; 1122: 447-456.
  58. Xiong Y, Xie LY, Abou-Samra AB. Signaling properties of the corticotropin-releasing factor (CRF) receptors: Decreased coupling efficiency of human type II CRF receptor. Endocrinology 1995; 136; 1828-1834.
  59. Iida-Klein A, Guo J, Xie LY, Juppner H, Potts Jr JT, Kronenberg HM, Bringhaurst FR, Abou-Samra AB, Segre GV Truncation of the carboxy-terminal region of the rat parathyroid hormone (PTH)/PTH-related peptide receptor enhances PTH stimulation of adenylyl cyclase but not phospholipase C. J. Biol. Chem 1995; 270;8458-8465.
  60. Schipani E, Weinstein LS, Bergwitz C, Iida-Klein A, Kong XF, Stuhrmann M, Kruse K, Whyte MP, Murray T, Schmidtke J, Van Dop C, Brickman AS, Crawford JD, Potts JT Jr, Kronenberg HM, Abou-Samra AB, Segre GV, Jüppner H. Pseudohypoparathyroidism type Ib is not caused by mutations in the coding exons of the human parathyroid hormone (PTH)/PTH-related peptide receptor gene. J Clin Endocrinol Metab 1995; 80:1611-1621.
  61. Jongen JWJM, Willemstein Van Hove EC, Van Der Meer JM, Bos MP, Jüppner H, Segre GV, Abou-Samra AB, Feyen JHM, Herrmann-Erlee MPM. Down-regulation of the receptor for parathyroid homrone and parathyroid hormone-related peptide by transfroming growth factor beta in primary fetat rat osteoblasts. Endocrinolgy 1995, 136:3260-3266.
  62. Guo J, Iida-Klein A, Huang X, Abou-Samra AB, Segre GV, Bringhaurst FR. PTH/PTHrP receptor density selectively modulates activation of phospholipase C and phosphate transport in LLCPK-1 cells. Endocrinology 1995; 136:3884-3891.
  63. Orloff JJ, Yulina K, Urena P, Schipani E, Vasavada R, Abou-Samra AB, Segre GV, Jüppner H. Further evidence for a novel receptor for amino-terminal parathyroid hormone-related protein on keratinocytes and squamous carcinoma cell lines. Endocrinology 1995 136:3016-3023.
  64. Nabhan C, Xiong Y, Xie LY, Abou-Samra AB. The alternatively-spliced type II corticotropin-releasing factor receptor, stably expressed in LLCPK-1 cells is not well coupled to the G protein. Biochem Biophys Res Comm 1995, 212:1015-1021.
  65. Yu J, Xie LY, Abou-Samra AB. Molecular cloning of a type A chicken corticotropin-releasing factor receptor with high affinity for urotensin I. Endocrinology, 1996, 137:192-197.
  66. Xie LY, Leung A, Segre GV, Yamamoto I and Abou-Samra AB. Dramatic downregulation of the PTH/PTHrP receptor by 1,25(OH)2 vitamin D in the osteoblast-like ROS 17/2.8 cells. Am J Physiol 1996, 270 (Endocrinol. Metab. 33):E654-E660.
  67. Lanske B, Karaplis AC, Lee K, Luz A, Vortkamp A, Pirro A, Karperien M, Defize LHK, Ho C, Mulligan RC, Abou-Samra AB, Juppner H, Segre GV, Kronenberg HK. PTH/PTHrP receptor in early development and indian hedgehog-regulated bone growth. Science 1996, 273:663-666.
  68. Jongen J W, Willemstein Van Hove EC, Van Der Meer JM, Bos MP, Juppner H, Segre GV, Abou-Samra AB, Feyen JH, Herrmann Erlee MP. Down-regulation of the receptor for parathyroid hormone (PTH) and PTH- related peptide by PTH in primary fetal rat osteoblasts. J Bone Miner Res 1996, 11:1218-25.
  69. Iida-Klein A, Guo J, Drake MT, Kronenberg HM, Abou-Samra AB, Bringhurst FR, Segre GV. Structural requirements of parathyroid hormone/parathyroid hormone- related peptide receptors for phospholipase C activation and regulation of phosphate uptake. Miner Electrolyte Metab 1995, 21:177-9.
  70. Joun H, Lanske B, Karperien M, Defize LHK and Abou-Samra AB. Tissure-specific transcritpion start site and alternative splicing of the PTH/PTHrP receptor gene: a new alternatively-spliced receptor that lacks the signal peptide. Endocrinology 1997, 138, 1742-1749.
  71. Iida-Klein A, Guo J, Takemura M, Drake MT, Potts Jr. JT, Abou-Samra AB, Bringhurst FR, Segre GV. Mutations in the second cytoplasmic loop of the rat parathyroid hormone (PTH)/PTH-related protein receptor result in selective loss of PTH-stimulated phospholipase C activity. J Biol Chem 1997, 272, 6882-6889.
  72. Qi LA, Leung AT, Xiong Y, Marx KA, Abou-Samra AB. Extracellular cysteines of the corticotropin releasing factor receptor are critical for ligand interaction. Biochemistry 1997, 36, 12442-12448.
  73. Qian F, Leung A, Abou-Samra AB. Agonist-dependent phosphorylation of the parathyroid hormone / parathyroid hormone-related peptide receptor. Biochemistry, 1998, 37, 6240-6246.
  74. Xie LY, Abou-Samra AB. Epitope-tag mapping of the extracellular and cytoplasmic domains of the rat PTH/PTHrP receptor. 1998, Endocrinology, 1998, 139, 4563-4567.
  75. Giannoukos G, Chilco PJ, Williams LJS, Abou-Samra AB. Characterization of an element within the rat parathyroid hormone / parathyroid hormone-related peptide receptor gene promoter that enhances expression in the osteoblastic osteosarcoma 17/2.8 cells. 1998, Biochem. Biophys. Res. Comm., 1999, 258, 336-340.
  76. Karperien M, Farih-Lips H, Hendriks JAA, Lanske B, Papapoulos SE, Abou-Samra AB, Lowik CWGM, Defize LHK. Identification of a retinoic acid-inducible element in the murine PTH/PTHrP receptor gene. Mol Endocrinol , 1999, 13, 1183-1196.
  77. Zhou A, Assil I, Abou-Samra AB. Role of Asparagine-Linked Oligosaccharides in the Function of the Rat PTH/PTHrP Receptor. Biochemistry, 2000, 39, 6514-6520.
  78. Williams LJS, Abou-Samra AB. The transcription factor SP1 and MAZ regulate expression of the parathyroid/parathyroid hormone related peptide receptor gene. J. Mol. Endocrinol 2001, 25, 309-319.
  79. Arai M, Assil I, Abou-Samra AB. Molecular Cloning of the Catfish Corticotropin Releasing Factor (CRF) Receptors: A third CRF receptor subtype that is prominent in pituitary and urophysis. Endocrinology, 2001, 142, 446-454.
  80. Assil I, Arai M, Shomali M, Abou-Samra AB. The juxta-membrane region of the amino-terminus of the corticotropin-releasing factor receptor type 1 is important for ligand interaction. Biochemistry, 2001, 40, 1187-1195.
  81. Assil I, Arai M, Shomali M, Abou-Samra AB. An oxidation-resistant radioligand for corticotropin-releasing factor receptors. Peptides, 2001, 22, 1055-1061.
  82. Assil I, Abou-Samra AB. N-glycosylation of the corticotropin-releasing factor receptor type 1 is important for its ligand specific interaction. Am J Phys (Endo Metab), 2001, E1015-E1021.
  83. Tawfeek H, Che J, Qian F, Abou-Samra AB. Parathyroid hormone receptor internalization is independent of protein kinase C and phospholipase c activation, Am J Phys (Endo Metab), 2001, 281, E545-E557.
  84. Tawfeek H, Qian F, Abou-Samra AB. Phosphorylation of the receptor for parathyroid hormone and parathyroid hormone-related peptide is required for internalization and regulates receptor signaling. Mol Endocrinol, 2002, 16, 1-13.
  85. Assil-Kishawi I and Abou-Samra AB. Sauvagine crosslinks to the second extracellular loop of the corticotropin-releasing factor type 1 receptor. J. Biol Chem 2002, 277:32558-32561.
  86. De Groef B, Geris KL, Manzano J, Bernal J, Millar RP, Abou-Samra AB, Porter TE, Iwasawa A, KuhnER, Darras VM. Involvement of thyrotropin-releasing hormone receptor, somatostatin receptor subtype 2 and corticotropin-releasing hormone receptor type 1 in the control of chicken thyrotropin secretion. Molecular and Cellular Endocrinology 2003, 203: 33- 39.
  87. Tawfeek H, Abou-Samra AB. An important role for the V-Type (H+)-ATPase and the Golgi Apparatus in the recycling of PTH/PTHrP receptor. Am J Phys (Endo Metab), 2004, 286(5):E704-10.
  88. Sneddon BW, Syme CA, Bisello A, Magyar CE, Rochdi MD, Parent JL, Weinman EJ, Abou-Samra AB, Friedman PA. Activation-independent parathyroid hormone receptor internalization is regulated by NHERF1 (EBP50). J Biol Chem 43787-43796, 278, 2003
  89. Zhu Q, Qi LJ, Shi A, Abou-Samra AB, Deth RC. Protein Kinase C Regulates 2A/D-Adrenoceptor Constitutive Activity. Pharmacology 71:80-90, 2004
  90. Bounoutas GS, Tawfeek H, Fröhlich LF, Chung UI and Abou-Samra AB. Impact of impaired receptor internalization on calcium homeostasis in knock-in mice expressing a phosphorylation-deficient parathyroid hormone (PTH) / PTH-related peptide receptor. Endocrinology 2006, 10:4674-4679
  91. Zalutskaya A, Arai M, Bounoutas GS and Abou-Samra AB. Impaired adaptation to repeated restraint and decreased response to cold in urocortin 1 knockout mice. Am J Physiol, Endo Metab 2007, 293: E259-263
  92. Tawfeek H and Abou-Samra AB. Negative Regulation of PTH-activated Phospholipase C by PTH/PTHrP Receptor Phosphorylation and Protein Kinase A Endocrinology 2008, 149:4016-4023
  93. Kolailatb R, Pettwayb GJ, McCauley LK, Abou-Samra AB, Datta NS. Regulation of mitogen-activated protein kinase phosphatase-1 by PTH/PTHrP involves cAMP and PKC pathways in differentiated osteoblasts.  Bone 2008, 42 (Supplement 1):S24-S25
  94. Assil-Kishawi I, Samra TA, Mierke D and Abou-Samra AB.  Residue 17 of sauvagine crosslinks to the first transmembrane domain of corticotropin-releasing factor receptor 1 (CRFR1).  J. Biol Chem 2008, in press.<

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Reviews and Books
  1. Abou-Samra AB, Fevre-Montange M, Borson F, Dechaud H, Tourniaire J. Circadian variation of immunoreactiveCRF in rat hypothalamus. In: Moody TW, ed. Neural and Endocrine Peptides and Receptors '85. New York: Plenum Press, 1986:149-157.
  2. Abou-Samra AB, Harwood JP, Catt KJ, Aguilera G. Mechanisms of action of CRF and other regulators of ACTH release. Ann NY Acad Sci. 1987; 512:67-86.
  3. Potts JT Jr, Nussbaum SR, Wiren K, Freeman M, Igarashi T, Okazaki T, Zajac J, Abou-Samra AB, Bringhurst FR, Segre GV, Kronenberg HM. Chemistry, molecular biology and mode of action of parathyroid hormone. In: Massery SG, Fujita T, eds. First International Conference on New Actions of Parathyroid Hormone, Kobe, Japan, October 26-31, 1987. New York: Plenum Press, 1989: 11-18.
  4. Segre GV, Abou-Samra AB, Jüppner H, Schipani E, Force T, Urena P, Freeman M, Kong XF, Kolakowski LF Jr, Hock J, Bonventre J, Potts JT Jr, Kronenberg HM. Characterization of cloned PTH/PTHrP receptors. J. Endocrinol. Invest. 1992, 15(supplement 6 to no. 9):11-17.
  5. Segre GV, Abou-Samra AB, Gardella TJ, Jüppner H, Kronenberg HM, Nussbaum SR, Potts JT Jr. Structural analysis of the PTH/PTHrP receptor's functional domains. In: Calcium Regulating Hormones and Bone Metabolism. Amsterdam: Elsevier Science Publishers BV, pp92-96.
  6. Kronenberg HM, Jüppner H, Abou-Samra AB, Potts JT, Jr, Segre GV. A common receptor for parathyroid hormone and parathyroid hormone-like peptide. In: Mornex R, Jaffiol C, Leclere J, eds. Proceedings of the Ninth International Congress on Endocrinology, Nice, France, 30 August-5 September 1992. Camforth, Lancs, UK: Parthenon Publishing, 1993:357-359.
  7. Abou-Samra AB, Juppner H, Kong XF, Schipani E, Iida-Klein A, Karga H, Urenea P, Gardella TF, Potts Jr JT, Kronenberg HM, Segre GV. Structure, fonction et expression de l’hormone parathyroidienne et du peptide apparenté à l’homone parathyroidienne. Actualite Nephrlogic Jean Hamburger, Hopital Necker 1993, pp185-201.
  8. Kronenberg HM, Bringhurst FR, Nussbaum SR, Jüppner H, Abou-Samra AB, Segre GV, Potts JT Jr. Parathyroid hormone: biosynthesis, secretion, chemistry, and action. In: Mundy GR, Martin TJ, eds. Handbook of Experimental Pharmacology (volume 107, 1993): Physiology and Pharmacology of Bone. Heidelberg, Germany: Springer-Verlag, pp507-567.
  9. Urena P, Abou-Samra AB, Juppner H, Kong XF, Lee K, Bringhaurst FR, Potts JTJr, Kronenberg HM, Segre GV. Mode d’action de l’hormone parathyroidienne (PTH) et du peptide apparenté à la PTH (PTHrP) au niveau des organs cibles. Les Annales d’Endocrinology (Paris) 1994, 55: 556-565.
  10. Abou-Samra AB, Juppner H, Kong XF, Schipani E, Iida-Klein A, Karga H, Urenea P, Gardella TF, Potts Jr JT, Kronenberg HM, Segre GV. Structure, Function and expression of the parathyroid hormone/parathyroid hormone receptor. Adv Nephrol 1994, 23:247-264.
  11. Juppner H, Schippanin E, Segre GV, Abou-Samra AB, Gardella T. Structure function studies with recombinant PTH/PTHrP receptors. J Bone Min Met 1994, 12 (Suppl 1): S169-S174.
  12. Juppner H and Abou-Samra AB Molecular Cloning of Peptide Hormone Receptors. In Principle of Bone Biology. (Ed: JP Bilezikian, LG Raisz and GA Rodan) Academic Press, pp1149-1160.
  13. Kong XF, Urena P, Schipani E, Jüppner H, Kronenberg HM, Potts JT Jr, Segre GV, Abou-Samra AB. Preliminary characterization of rat genomic clones encoding the common receptor for parathyroid hormone and parathyroid hormone related peptide. In: Calcium Regulating Hormones and Bone Metabolism. Amsterdam: Elsevier Science Publishers BV, pp97-101 (1993).
  14. Jüppner H, Abou-Samra AB. Molecular cloning of peptide hormones and their receptors. In: Bilezikian JP RL, Rodan GA, ed. Principles of Bone Biology. San Diego: Academic Press, 1996:1149-1159.
  15. Kronenberg H, Abou-Samra A, Bringhurst F, Gardella T, Jüppner H, Segre G. The PTH/PTHrP receptor: One receptor for two ligands. In: Thakker R, ed. Genetics of Endocrine and Metabolic Disorders. London: Chapman & Hall, 1997:389-420.
  16. Abou-Samra AB Parathyroid Hormone and Its Receptors. In Advances in Organ Biology: Molecular and cellular biology of bone. (Ed: Moniz), 1998 (Volume 5A):161-185.
  17. Demay M, Jüppner H, Abou-Samra AB, Segre GV, Kronenberg HM. Parathyroid hormone biosynthesis and action: molecular analysis of the PTH gene and PTH/PTHrP receptor. In: Noda M, ed. Molecular and Cellular Biology of the Bone. San Diego, California: Academic Press,
  18. Kronenberg HM, Abou-Samra AB, Jüppner H, Potts JT Jr, Segre GV. Characterization of a receptor that binds both PTH and PTHrP. In: Calcium Regulating Hormones and Bone Metabolism. Amsterdam: Elsevier Science Publishers BV, pp79-82.
Patents
  1. Abou-Samra AB, Juppner H, Kronenberg HM, Potts JT, Schipani E, Segre GV. DNA and vectors encoding the parathyroid hormone receptor, transformed cells, and recombinant protein. US PATENT #5494806 issued 2/27/1996
  2. Abou-Samra AB, Juppner H, Kronenberg HM, Potts JT, Schipani E, Segre GV. Parathyroid hormone receptor and DNA encoding same. US PATENT #5840853 issued 11/24/1998
  3. Abou-Samra AB, Juppner H, Kronenberg HM, Potts JT, Schipani E, Segre GV.. Parathyroid hormone receptor. US PATENT # 5886148 issued 3/23/1999
  4. Segre GV, Kronenberg HM, Abou-Samra AB, Juppner H, Potts JT, Schipani E. DNA encoding parathyroid hormone receptor. US PATENT #10267730 issued 11/7/2006
  5. Segre GV, Kronenberg HM, Abou-Samra AB, Juppner H, Potts JT, Schipani E. Parathyroid hormone receptor binding method. US PATENT #7150974 issued 12/19/2006